A9469

Sigma

 

Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Synonym:Monoclonal ANTI-FLAG® M2 antibody produced in mouse

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Description

ApplicationFor simple, one-step detection by ELISA, dot blot or Western blotting. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity.
Other NotesTo view FLAG® technology literature, visit www.sigma-aldrich.com/flagliterature.
SpecificityBinds to the FLAG® epitope wherever it is located in the fusion protein: amino-terminal, Met-amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent.
Physical formSolution in Tris buffered saline containing 50% glycerol plus stabilizer and preservative
Legal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
 FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

antibody formpurified immunoglobulin
cloneM2 (monoclonal)
formbuffered aqueous glycerol solution
application(s)indirect ELISA: 1:20,000
isotypeIgG1
shipped inwet ice
storage temp.−20°C

Safety

Hazard CodesXn
Risk Statements21/22
Safety Statements36
WGK Germany1